Cell to cell communication by biophotons has been demonstrated in plants, bacteria, animal neutrophil granulocytes and kidney cells. Whether such signal communication exists in neural cells is unclear. By developing a new biophoton detection method, called in situ biophoton autography (IBA), we have investigated biophotonic activities in rat spinal nerve rootsin vitro. We found that different spectral light stimulation (infrared, red, yellow, blue, green and white) at one end of the spinal sensory or motor nerve roots resulted in a signiﬁcant increase in the biophotonic activity at the other end. Such effects could be signiﬁcantly inhibited by procaine (a regional anaesthetic for neural conduction block) or
classic metabolic inhibitors, suggesting that light stimulation can generate biophotons that conduct along the neural ﬁbers, probably as neural communication signals. The mechanism of biophotonic conduction along neural ﬁbers may be mediated by protein–protein biophotonic interactions. This study may provide a better understanding of the fundamental mechanisms of neural communication, the functions of the nervous system, such as vision, learning and memory, as well as the mechanisms of human neurological diseases.